CASP3 (Caspase3 Apoptosis-Related Cysteine Protease), also known as YAMA, CPP32 or APOPAIN, is a caspase protein that interacts with caspase 8 and caspase 9.
It is encoded by the CASP3 gene.
The CASP3 protein is a member of the cysteine-aspartic acid protease (caspase) family.
Tiso et al.
(1996) used radiation hybrid mapping to localize the CPP32 gene to human chromosome 4q33-q35.1.
Fernandes-Alnemri et al.
(1994) found that overexpression of CPP32 in insect cells induced apoptosis.
Coexpression of the 2 CPP32 subunits in insect cells also resulted in apoptosis.
Tewari et al.
(1995) showed that purified human ICE cleaved the Yama proenzyme into a proteolytically active form and that activated Yama cleaved PARP into the 85-kD apoptotic form.
UniProt Number:P70677
Gene ID:CASP3
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes mouse and rat mr.CASP3(p17). No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the N-terminal of mouse CASP3(p17), different from the related rat sequence by one amino acid.
