BUB3(BUDDING UNINHIBITED BY BENZIMIDAZOLES 3) is a protein that in humans is encoded by the BUB3 gene.
And it is mapped on 10q26.13.Bub3 is also a protein involved with the regulation of the Spindle Assembly Checkpoint (SAC); though BUB3 is non-essential in yeast, it is essential in higher eukaryotes.
As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase to achieve biorentation.
Bub3 directs the localization of Bub1 at the kinetochore in order to activate the SAC.
Bub3 also acts as a regulator in that it affects binding of Mad3 to Mad2.
Using deletion analysis, the authors identified a domain of BUB1 that is required both for binding BUB3 and for kinetochore localization of BUB1.
UniProt Number:O43684
Gene ID:BUB3
Applications:Suitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen), and Immunocytochemistry.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat BUB3. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence in the middle region of human BUB3, identical to the related rat and mouse sequences.
