84-2097-64　MBD4 (MED1, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase) 100ug　144882

特徴

• MBD4 (Methyl-CpG-Binding Domain Protein 4), also known as MED1, is a protein that in humans is encoded by the MBD4 gene.
• MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells (Hendrich and Bird, 1998).
• Riccio et al.
• (1999) mapped the MBD4 gene to chromosome 3q21-q22 by FISH.
• Hendrich and Bird (1998) found that both MBD2 and MBD4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo.
• They concluded that MBD2 and MBD4 are likely to be mediators of the effects of DNA methylation in mammalian cells.
• Hendrich et al.
• (1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro.
• Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.
• UniProt Number:O95243
• Gene ID:MBD4
• Applications:Suitable for use in Western Blot.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Handling:Store at -20˚C for one year.
• After reconstitution, store at 4˚C for one month.
• Can also be aliquoted and stored frozen at -20˚C for long term
• Avoid repeated freezing and thawing.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.