MAP2K6 (Mitogen-activated protein kinase kinase 6), also known as MAP kinase kinase 6 (MAPKK 6) or MAPK/ERK kinase 6 is an enzyme that in humans is encoded by the MAP2K6 gene, on chromosome 17.
MAPKK 6 is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase.
Han et al.
(1996) described experiments addressing the function of MKK6 in intracellular signaling pathways leading toward activation of p38 MAPK.
Mukherjee et al.
(2006) found that the Yersinia YopJ virulence factor acted as an acetyltransferase, using coenzyme A to modify critical ser and thr residues in the MAP2K6 activation loop, thereby blocking phosphorylation and kinase activation.
They proposed that ser and/or thr acetylation by a bacterial effector may be a mechanism that occurs in other signaling pathways.
UniProt Number:P52564
Gene ID:MAP2K6
Applications:Suitable for use in Western Blot.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat MAP2K6. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the N-terminal of human MAP2K6, identical to the related mouse sequence and different from the related rat sequence by one amino acid.
