Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene.
The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane.
The lamin family of proteins make up the matrix and are highly conserved in evolution.
During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated.
Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression.
Vertebrate lamins consist of two types, A and B.
This gene encodes one of the two B type proteins, B1.
UniProt Number:P20700
Gene ID:LMNB1
Applications:Suitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen), and Immunocytochemistry.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human and rat Lamin B1. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human Lamin B1, different from the related rat sequence by one amino acid, mouse sequence by three amino acids.
