KLF8 (Krueppel-like factor 8) is a protein that in humans is encoded by the KLF8 gene.
It is also named as ZNF741, or BKLF3.
Kruppel-like factor proteins, including KLF8, all contain a characteristic C-terminal DNA-binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers.
The International Radiation Hybrid Mapping Consortium mapped the KLF8 gene to the X chromosome (WI-18614).
Suske et al.
(2005) stated that the human KLF8 gene maps to chromosome Xp11.21, and the mouse Klf8 gene to chromosome XF3.
van Vliet et al.
(2000) identified a cDNA encoding KLF8, also called ZNF741.
Expression of KLF8 or only its zinc fingers showed that the fingers could bind to a CACCC box.
Sequence analysis predicted that the 359-amino acid protein contains a nuclear localization signal upstream of the zinc fingers, a valine-rich hydrophobic stretch, and a pro-val-asp-leu-ser (PVDLS) motif.
Yeast 2-hybrid and GST pull-down analysis demonstrated that CTBP2 interacts with KLF8 through the PVDLS motif.
UniProt Number:O95600
Gene ID:KLF8
Applications:Suitable for use in Western Blot.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human KLF8. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide corresponding to a sequence at the N-terminal of human KLF8.
