KLF5 (Krueppel-like factor 5) is a protein that in humans is encoded by the KLF5 gene.
It is also known as BASIC TRANSCRIPTION ELEMENT-BINDING PROTEIN 2 or BTEB2.
This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins.
Suske et al.
(2005)stated that the human KLF5 gene maps to chromosome 13q21.33, and the mouse Klf5 gene to chromosome 14E2.1.
Sogawa et al.
(1993)isolated a cDNA encoding BTEB2.
The predicted 219-amino acid protein contains 3 consecutive zinc finger motifs near the C terminus.
The zinc finger domains were 59% and 64% identical to those of Sp1 (189906) and BTEB1, respectively.
Both BTEB2 and Sp1 have a short basic region preceding the zinc finger motifs that may play an auxiliary role in DNA binding.
The N-terminal region of BTEB2 is proline rich.
Recombinant BTEB2 protein and Sp1 showed very similar DNA-binding specificity in vitro.
When expressed in mammalian cells, BTEB2 activated the transcription of a reporter gene fused to a GC box-containing promoter.
Sogawa et al.
(1993) also identified the rat BTEB2 homolog.
Northern blot analysis of rat tissues revealed that BTEB2 is expressed only in the testis and placenta.
UniProt Number:Q13887
Gene ID:KLF5
Applications:Suitable for use in Western Blot.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat KLF5. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence in the middle region of human KLF5, identical to the related rat and mouse sequences.
