KLF3(Krueppel-like factor 3) is a protein that in humans is encoded by the KLF3 gene.
It is also known as BKLF(Basic kruppel-like factor).
KLF3 is a member of the Krüppel-like factors family of transcription factors.
KLF3 primarily represses transcription through the transcriptional corepressor C-terminal binding protein (CtBP).
Suske et al.
(2005) stated that the human KLF3 gene maps to chromosome 4p14, and the mouse Klf3 gene maps to chromosome 5C3.3.
Suske et al.
(2005) stated that the human and mouse KLF3 proteins contain 345 and 344 amino acids, respectively.
Both have a characteristic zinc finger domain, but lack the N-terminal buttonhead box found in related SP transcription factors.
The repression mediated through KLF3 is also partially dependent on the sumoylation of its K10 and K197.
Within KLF3's C-terminal, which is highly conserved throughout the KLF family, resides three C2H2 zinc fingers that preferentially bind the site NRG GNG NGR.
UniProt Number:P57682
Gene ID:KLF3
Applications:Suitable for use in Western Blot.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat KLF3. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the N-terminal of human KLF3, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
