IGF1R(Insulin-like Growth Factor 1 (IGF-1) Receptor) is a protein found on the surface of human cells.
It is a transmembrane receptor that is activated by a hormone called Insulin-like growth factor 1 (IGF-1) and by a related hormone called IGF-2.
It belongs to the large class of tyrosine kinase receptors.
The IGF1R gene is mapped on 15q26.3.
IGF-1 plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues.
Using a yeast 2-hybrid system, Dey et al.
(1998) identified a regulatory subunit of phosphatidylinositol (PI) 3-kinase, PIK3R3, as a binding partner of IGF1R.
Functional interaction between BRCA1 and SP1 in the regulation of the IGF1R gene was studied in Schneider cells, a Drosophila cell line which lacks endogenous SP1.
In these cells, BRCA1 suppressed 45% of the SP1-induced trans-activation of the IGF1R promoter.
Overexpression of the Grb10-binding fragment of Gigyf1 resulted in a significant increase in Igf1-stimulated Igf1r tyrosine phosphorylation.
Like the insulin receptor, the IGF-1 receptor is a receptor tyrosine kinase - meaning it signals by causing the addition of a phosphate molecule on particular tyrosines.
IGF-1 activates the Insulin receptor at approximately 0.1x the potency of insulin.
Part of this signaling may be via IGF1R-InsulinReceptor heterodimers.
UniProt Number:P08069
Gene ID:IGF1R
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide, Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human, mouse and rat IGF1R. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide mapping to a fragment of residues within amino acids 1353-1367 in the C-terminal region of human IGF1R, different from the related rat and mouse sequences by one amino acid.
