HSPA8(heat shock 70kDa protein 8) also known as HSC70, HSC71, HSP73,HSPA10, FORMERLY , LAP1 or LPS-ASSOCIATED PROTEIN 1, is a heat shock protein that in humans is encoded by the HSPA8 gene.
The HSPA8 gene contains 9 exons and spans 5 kb.
The deduced HSPA8 protein has 646 amino acids and a predicted molecular mass of 70,899 Da.
The HSPA8 gene is mapped on 11q24.1.
HSPA8 plays an important role in cells by transiently associating with nascent polypeptides to facilitate correct folding.
HSP73 also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell.
Rapid decay involves AU-rich binding protein AUF1, which complexes with heat-shock proteins HSC70 and HSP70, translation initiation factor EIF4G, and poly(A)-binding protein.
In the absence of Il3, Hsc70 formed a complex with Hsp40 and Hip, and this complex, in association with Eif4g and Pabp, formed a high-stability complex with Bim mRNA that protected it from ribonucleases.
UniProt Number:P11142
Gene ID:HSPA8
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat HSPA8. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human HSPA8, different from the related mouse and rat sequences by one amino acid.
