The Hsp40 (heat shock protein with molecular size of approximately 40 kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein.
Ohtsuka (1993) isolated a cDNA encoding a 40-kD heat-shock protein designated HSPF1.
The deduced 340-amino acid HSPF1 protein is 34% identical to E.
coli DnaJ and 34% and 36% identical to HSJ1 and HSJ2, respectively.
HSPF1 gene spans over 7 kb and contains 3 exons and 2 introns.
HSPF1 gene is mapped to chromosome 19p13.2.
UniProt Number:P25685
Gene ID:DNAJB1
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity:Recognizes human, mouse and rat HSP40. No crossreactivity with other proteins.
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:A synthetic peptide corresponding to a sequence at the C-terminal of human HSP40, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.