84-2095-98　Heat shock cognate 71kD protein (HSP70s) 100ug　144653

特徴

• The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins.
• The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress.
• All of the Hsp70 proteins have three major functional domains: An N-terminal ATPase domain binds ATP (Adenosine triphosphate) and hydrolyzes it to ADP (Adenosine diphosphate); A substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues; A C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain.
• By binding tightly to partially-synthesized peptide sequences (incomplete proteins), Hsp70 prevents them from aggregating and being rendered nonfunctional.
• And it also can act to protect cells from thermal or oxidative stress.
• Finally, Hsp70 seems to be able to participate in disposal of damaged or defective proteins.
• Interaction with CHIP (Carboxyl-terminus of Hsp70 Interacting Protein)–an E3 ubiquitin ligase–allows Hsp70 to pass proteins to the cell's ubiquitination and proteolysis pathways.
• UniProt Number:E9PSH5
• Gene ID:HSPA8
• Applications:Suitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen), and Immunocytochemistry.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Handling:Store at -20˚C for one year.
• After reconstitution, store at 4˚C for one month.
• Can also be aliquoted and stored frozen at -20˚C for long term
• Avoid repeated freezing and thawing.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.