HDAC3 (HISTONE DEACETYLASE 3) is a member of the histone deacetylase/acuc/apha family of proteins that is an enzyme that in humans is encoded by the HDAC3 gene.
The HDAC3 gene is mapped to 5q31.3.
HDAC3 has histone deacetylase activity and represses transcription when tethered to a promoter.
It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1.
The protein can also down-regulate p53 function and thus modulate cell growth and apoptosis.
And this gene is regarded as a potential tumor suppressor gene.
HDAC3 has an open reading frame of 428 amino acids and shares 53% amino acid identity with HDAC1 and 52% with HDAC2.
The catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor NCOR2.
All experimental conditions leading to the suppression of HDAC4 binding to NCOR2 and to HDAC3 resulted in loss of enzymatic activity associated with HDAC4.
HDAC3 recruitment to the genome displays a circadian rhythm in mouse liver.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Heat induced antigen retrievalImmunohistochemistry (Frozen): 0.5-1.0ug/mlImmunocytochemistry: 0.1-1.0ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability: Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O
Specificity：Recognizes human HDAC3. Species Crossreactivity: mouse and rat No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to aa411-428, NEFYDGDHDNKESDVEI at the C-terminal of human HDAC3, identical to the related rat and mouse sequences.
