MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene.
Mfge8 is secreted protein found in vertebrates, including mammals as well as birds.
By fluorescence in situ hybridization, Collins et al.
(1997) mapped the MFGE8 gene to chromosome 15q25.
Hanayama et al.
(2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes.
MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine.
MFGE8, when engaged by phospholipids, bound to cells via its RGD motif.
It bound particularly strongly to cells expressing alpha-V-beta-3 integrin.
Bu et al.
(2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice.
Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.
UniProt Number:Q08431
Gene ID:MFGE8
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human h.MFGE8. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide corresponding to a sequence in the middle region of human MFGE8.
