EIF2AK3 (Eukaryotic Translation Initiation Factor 2-Alpha Kinase 3), also called PEK, is an enzyme that in humans is encoded by the EIF2AK3 gene.
By fluorescence in situ hybridization and radiation hybrid analysis, Hayes et al.
(1999) mapped the EIF2AK3 gene to chromosome 2p12.Harding et al.
(2000) reported that a targeted mutation of the mouse Eif2ak3 gene, which they called Perk, abolished the phosphorylation of eIF2-alpha in response to accumulation of malfolded proteins in the ER, resulting in abnormally elevated protein synthesis and higher levels of ER stress.
Using a library of endoribonuclease-prepared short interfering RNAs (esiRNAs), Kittler et al.
(2004) identified 37 genes required for cell division, one of which was EIF2AK3.
UniProt Number:Q9NZJ5
Gene ID:EIF2AK3
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human h.EIF2AK3. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide corresponding to a sequence at the N-terminal of human EIF2AK3.
