EIF2AK2 (Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2), also called PKR, is an enzyme that in humans is encoded by the EIF2AK2 gene.
Activation of EIF2AK2 allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2, leading to the inhibition of protein synthesis (summary by Kuhen et al., 1996).
By FISH analysis, Squire et al.
(1993) assigned the EIF2AK2 gene to the boundary between chromosome 2p22-p21.
Ben-Asouli et al.
(2002) showed that human gamma-interferon mRNA uses local activation of PKR in the cell to control its own translation yield.
IFNG mRNA was found to activate PKR through a pseudoknot in its 5-prime untranslated region.
Taylor et al.
(1999) studied the mechanism underlying the resistance of hepatitis C virus (HCV) to interferon.
They demonstrated that the HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PKR and the translation initiation factor EIF2-alpha, a target of PKR.
E2 inhibited the kinase activity of PKR and blocked its inhibitory effect on protein synthesis and cell growth.
UniProt Number:P19525
Gene ID:EIF2AK2
Applications:Suitable for use in Western Blot.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human h.EIF2AK2. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide corresponding to a sequence at the N-terminal of human EIF2AK2.
