Cystatin B(CSTB),also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors.
It has been isolated from human spleen and liver and its amino acid sequence has been fully determined.
The cystatin B gene is located on 21q22.3.
It is widely distributed and is localized mostly intracellularly, but has been found extracellularly.
The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b.
Its role is thought to be as a protector against the proteinases leaking from lysosomes.
A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1.
Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.
UniProt Number:P04080
Gene ID:CSTB
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human h.CSTB. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide corresponding to a sequence in the middle region of human CSTB.
