84-2095-43　GDNF (Astrocyte-derived trophic factor) 100ug　144567

特徴

• GDNF, Glial cell-derived neurotrophic factor, is a protein that, in humans, is encoded by the GDNF gene.
• GDNF is a small protein that potently promotes the survival of many types of neurons.
• GDNF is a founding member of the GDNF family of ligands (GFL).The GDNF gene is mapped to human chromosome 5p13.1-p12 by fluorescence in situ hybridization (FISH).
• This gene encodes a highly conserved neurotrophic factor.
• The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosisof motor neurons induced by axotomy.
• The encoded protein is processed to a mature secreted form that exists as a homodimer.
• The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (Paraffin): 0.5-1ug/ml.
• Antigen retrieval by boiling paraffin sections in 10mM citrate buffer, pH 6.0 for 20 minutess is required for the staining of formalin/paraffin sections
• Immunocytochemistry: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.