Interferons induce a large number of genes in their target cells, including those coding for guanylate-binding proteins (GBPs).
GBPs, such as GBP1, are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP) and are distinguished from the GTP-binding proteins by the presence of 2 binding motifs rather than 3 .
The 593-amino acid GBP1 protein shares 77% and 88% identity with GBP2 and GBP3 , respectively.
All GBPs, including GBP1, have a conserved N-terminal globular GTP-binding domain containing 2 consensus sequences and a third T(L/V)RD sequence not found in other GTPases.
The GBP1 gene to the GBP gene cluster on chromosome 1p22.2.
It is located telomeric to GBP2 and centromeric to GBP3.
High expression of GBP1, GBP2, and GBP3 are in endothelial cells after stimulation with IFNG , TNF , or IL1B .
UniProt Number:P32455
Gene ID:GBP1
Applications:Suitable for use in Western Blot.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human GBP1. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human GBP1.
