84-2095-12　［受注停止］FGF1 (Acidic fibroblast growth factor) 100ug　144518

特徴

• Fibroblast growth factor 1 (acidic), also known as FGF1/ECGF/HBGF1, is a human gene which is mapped to 5q31.
• Human FGF1 shares 96% amino acid sequence homology with both rat and mouse.
• The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family.
• FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.
• This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor.
• It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.
• Additionally, Acidic fibroblast growth factor is derived from beta-endothelial cell growth factor (ECGFB) by posttranslational processing.
• Alpha-ECGF is also derived from ECGFB in the same manner.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications have not been tested.
• Recommended Dilutions:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (Paraffin): 0.5-1ug/ml.
• Boiling the paraffin sections in 10mM citrate buffer, pH 6 for 20 minutes is required for the staining of formalin/paraffin sections
• ELISA: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.