Fascin is a actin cross-linking protein.The Fascin gene contains 5 exons and spans 7 kb.
It is a 54-58 kilodalton monomeric actin filament bundling protein originally isolated from sea urchin egg but also found in Drosophila and vertebrates, including humans.
Fascin (from the Latin for bundle) is spaced at 11 nanometre intervals along the filament.
The bundles in cross section are seen to be hexagonally packed, and the longitudinal spacing is compatible with a model where fascin cross-links at alternating 4 and 5 actins.
It is calcium insensitive and monomeric.
Fascin binds beta-catenin, and colocalizes with it at the leading edges and borders of epithelial and endothelial cells.
The role of Fascin in regulating cytoskeletal structures for the maintenance of cell adhesion, coordinating motility and invasion through interactions with signalling pathways is an active area of research especially from the cancer biology perspective.
Abnormal fascin expression or function has been implicated in breast cancer, colon cancer, esophageal squamous cell carcinoma, gallbladder cancer and prostate cancer.
UniProt Number:Q16658
Gene ID:FSCN1
Applications:Suitable for use in Western Blot.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat Fascin. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the N-terminal of human Fascin, identical to the related rat and mouse sequences.
