84-2094-97　Erythropoietin (EPO) 100ug　144495

特徴

• EPO, Erythropoietin, is an acidic glycoprotein hormone with a molecular mass of 34 kD.
• It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow.
• This gene is mapped to 7q22.1.
• It is produced by interstitial fibroblasts in the kidney in close association with peritubular capillary and tubular epithelial cells.
• It is also produced in perisinusoidal Ito cells in the liver.
• While liver production predominates in the fetal and perinatal period, renal production is predominant during adulthood.
• As the prime regulator of red cell production, its major functions are to promote erythroid differentiation and to initiate hemoglobin synthesis.
• It also has other known biological functions.
• For example, erythropoietin plays an important role in the brain's response to neuronal injury.
• EPO is also involved in the wound healing process.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlImmunohistochemistry (Paraffin): 0.5-1ug/ml.
• Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 minutes is required for staining of formalin/parffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile dH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.