Estrogen receptor-beta, referred to as ESR2, is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses.
This gene is mapped to 14q and comprises 8 exons spanning approximately 40 kb.
ESR2 is expressed in multiple tissues, including developing spermatids of the testis and in ovarian granulosa cells1.
ESR-beta is homologous to the previously identified ESR-alpha and has an overlapping but nonidentical tissue distribution.
The DNA-binding domain of ESR-beta is 96% conserved compared to ESR, and the ligand-binding domain shows 58% conserved residues.
ESR-beta is expressed in human thymus, spleen, ovary, and testis2.
Rat ESR-beta is expressed in rat prostate and ovary and is homologous to rat ESR (95% conserved DNA-binding domain; 55% conserved ligand-binding domain)3.
ESR2 mRNA was coexpressed with ESR1 and its splice variants in 60% of prolactinomas, 100% of mixed growth hormone /prolactin tumors, and 29% of gonadotroph tumors.
ESR2 gene expression was not limited to ESR1-positive tumor subtypes, however, and was also found in 100% of null cell tumors, 80% of somatotroph tumors, and 60% of corticotroph tumors4.
UniProt Number:Q92731
Gene ID:ESR2
Applications:Suitable for use in Western Blot.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human ER beta. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide corresponding to a sequence at the N-terminal of human ERbeta, different from the related rat and mouse sequences by four amino acids.
