EIF6 (Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene.
By fluorescence in situ hybridization, Sanvito et al.
(1998) mapped the ITGB4BP gene to 20q11.2.
Ceci et al.
(2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6.
In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits.
Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C.
Gandin et al.
(2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo.
Eif6-null mouse embryos were lethal at preimplantation.
Heterozygous mice had 50% reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.
UniProt Number:P56537
Gene ID:EIF6
Applications:Suitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human,mouse and rat EIF6. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence in the middle region of human EIF6, different from the related rat and mouse sequences by one amino acid.
