Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is responsible for maintaining low intracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA.
dUTPase activity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and dUTPase plays a role in DNA nucleotide metabolism.
This protein, present predominantly in the cytoplasm, contains 252 amino acids with a Mr of 26,704.
It exhibits 35% identity with the E.
coli dUTPase and 53% identity with the Saccharomyces cerevisiae enzyme.
The nuclear and mitochondrial forms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the use of alternative 5-prime exons.
Human dUTPase exhibits 92% identity with rat.
Moreover, this enzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis.
UniProt Number:P33316
Gene ID:DUT
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat dUTPase. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human dUTPase, different from the related rat and mouse sequences by two amino acids.
