The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins.
The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins.
By radiation hybrid analysis, Castrillon et al.
(2000) mapped the VASA gene to 5q.
By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12.
This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog (Ddx4) resides (Abe and Noce, 1997).
Using a combination of proteomics, cytology, and functional analysis in C.
elegans, Chu et al.
(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.
UniProt Number:Q9NQI0
Gene ID:DDX4
Applications:Suitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen), and Immunocytochemistry.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat DDX4. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence in the middle region of human DDX4, identical to the the related rat and mouse sequences.
