DCC(Deleted IN Colorectal Carcinoma) also known as CRC18 or CRCR1.
The DCC gene encodes a functional receptor for netrin and mediates axon outgrowth and the steering response.
Heterozygous loss-of-function mutations in DCC can result in congenital mirror movements.
Alterations in DCC occur frequently in colorectal cancer.
Studying a YAC contig containing the entire DCC coding region, they showed that the DCC gene spans approximately 1.4 Mb.
Vogelstein (1995) stated that the precise location of the DCC gene was thought to be 18q21.3.
DCC is a receptor or a component of a receptor that mediates the effects of netrin-1 on commissural axons, and they complement genetic evidence for interactions between DCC and netrin homologs in C.
DCC protein could be detected in varying abundance in all specimens of normal colonic mucosa analyzed as well as in all specimens of adenomatous polyps, colorectal carcinoma and colorectal liver metastases.
DCC may function as a tumor-suppressor protein by inducing apoptosis in settings in which ligand is unavailable (for example, during metastasis or tumor growth beyond local blood supply) through functional caspase cascades by a mechanism that requires cleavage of DCC at asp1290.
Stein et al.
(2001) concluded that DCC plays a central role in netrin signaling of axon growth and guidance independent of A2B receptor activation.
UniProt Number:P43146
Gene ID:DCC
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat DCC. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the N-terminal of human DCC, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
