DAXX(death-domain associated protein) also known as DAP6(Death-associated protein 6) or BING2, was first discovered through its cytoplasmic interaction with the classical death receptor Fas.
Human DAXX encodes a 740-amino acid polypeptide containing a nuclear localization signal.
Functional analyses by Yang et al.
(1997) demonstrated that Daxx binds to the Fas death domain and enhances Fas-mediated apoptosis.
The authors suggested that DAXX and FADD define 2 distinct apoptotic pathways downstream of Fas.
The DAXX gene is mapped to human chromosome 6p21.3 by somatic cell hybrid panels and fluorescence in situ hybridization, a region containing the HLA and putative autoimmune disease genes.
Immunoprecipitation and Western blot analysis with DAXX mutants showed that the N terminus of DAXX interacts with the C terminus of DMAP.
Transient expression of DAXX or DMAP1 caused repression of glucocorticoid receptor-mediated transcription.
UniProt Number:Q9UER7
Gene ID:DAXX
Applications:Suitable for use in Western Blot.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat DAXX. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence in the middle region of human DAXX, identical to the related mouse and rat sequences.
