CXCL4, Chemokine (C-X-C motif) ligand 4, is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin.
CXCL4 belongs to the CXC chemokine family.
The CXCL4 gene contains 3 exons spanning approximately 1 kb.
The gene is encoded on a 10-kb EcoRI fragment of genomic DNA.
By in situ hybridization, the CXCL4 gene is mapped to chromosome 4q12-q21.
Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation.
As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (Paraffin): 0.5-1ug/ml Requires HIER using sodium citrate buffer pH6.0 prior to staining
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human CXCL4. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC N WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide corresponding to aa85-101 DLQAPLYKKIIKKLLES, at the C-terminal of human CXCL4.
