Cullin-3(Cul 3), is a protein that in humans is encoded by the CUL3 gene.
Kipreos et al.
(1996) identified a conserved gene family, designated cullins, with at least 5 members in nematodes, 6 in humans, and 3 in S.
cerevisiae.
Human CUL3 is an ortholog of nematode cul3.
Hartz (2012) mapped the CUL3 gene to chromosome 2q36.2 based on an alignment of the CUL3 sequence with the genomic sequence (GRCh37).
Sumara et al.
(2007) found that KLHL9, KLHL13, and CUL3 interacted directly in a 370-kD protein complex in HeLa cell lysates.
Rondou et al.
(2008) showed that interaction between KLHL12 and the CUL3 ubiquitin ligase complex directed ubiquitination of dopamine receptor D4 (DRD4).
KLHL12 interacted directly with CUL3 and with the polymorphic intracellular loop-3 of D4.
By immunoprecipitation analysis of HeLa cells and HCT116 human colon carcinoma cells expressing epitope-tagged proteins, Kigoshi et al.
(2011) showed that KLHL7 interacted with CUL3 and ROC1 (RBX1).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human Cullin3. Species Crossreactivity: rat. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：A synthetic peptide corresponding to aa426-444, DVFERYYKQHLARRLLTNK in the middle region of human Cullin 3 (Q13618). Species Sequence Homology: mouse
