CTBP1, C-terminal-binding protein 1, is a protein that in humans is encoded by the CTBP1 gene.
The CtBP1 protein binds to the C-terminus of adenovirus E1A proteins.
This gene is mapped to 4p16.This phosphoprotein is a transcriptional repressor (corepressor) and may play a role during cellular proliferation.
This protein and the product of a second closely related gene, CTBP2, can dimerize.
CtBP1 and CtBP2 preferentially associate with the E1A via a 5 amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation.
CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis.
During skeletal and T cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of δEF1, a cellular zinc finger-homeodomain protein, and thereby enhances δEF1-induced transcriptional silencing.
In addition, CtBP complexes with CtIP, a 125 kDa protein that recognizes distinctly different protein motifs from CtBP.
CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity.
Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development.
Alternative splicing of transcripts from this gene results in multiple transcript variants.
UniProt Number:Q13363
Gene ID:CTBP1
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat CTBP1. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human CTBP1, different from the related rat and mouse sequences by one amino acid.
