CRKL(v-crk sarcoma virus CT10 oncogene homolog (avian)-like) also called ONCOGENE CRKL, encodes a 303-amino acid polypeptide with a predicted molecular mass of 36 kD.
While CRKL is not the human homolog of CRK, it is similar to protein-tyrosine kinases with SH2 and SH3 (src homology) domains.
By fluorescence in situ hybridization, the CRKL gene is localized to chromosome 22q11, centromeric of the chronic myelogenous leukemia breakpoint region.
Senechal et al.
(1996) showed that CRKL becomes phosphorylated when overexpressed, activates Ras-dependent and JNK pathways, and transforms fibroblasts.
The authors also found CRKL to be a substrate for the BCR-ABL tyrosine kinase, leading them to conclude thatCRKL is a tyrosine kinase and an oncogene.
Crkl was required for normal cellular responses to Fgf8, including survival and migration, Erk activation, and target gene expression.
UniProt Number:P46109
Gene ID:CRKL
Applications:Suitable for use in Western Blot.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat CRKL. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human CRKL, different from the related mouse and rat sequences by one amino acid.
