Connexin26(CX26), also known as GAP junction protein, beta2, GJB2.
Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells.
These structures were shown to consist of cell-to-cell channels.
Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ.
The 3-prime untranslated region of the CX26 transcript contains a putative mRNA instability sequence.
The deduced 226-amino acid protein has a calculated molecular mass of about 26 kD.
CX26 shares 92.5% identity with rat Cx26.
connexin 26 (GJB2) is assigned to human chromosome 13q11-q12 .Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response.
Connexin 26 gene (GJB2) mutation modulates the severity of hearing loss associated with the 1555A-G mitochondrial mutation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human Connexin 26. Species Crossreactivity: rat No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa1-16, MDWGTLQTILGGVNKH at the N-terminal of human Connexin 26. This sequence differs from the related mouse and rat sequences by two amino acids.
