Cytokeratin 8 (CK8), also known as Keratin 8 (K8), is a type II keratin.
Among the various intermediate filament (IF) proteins, cytokeratin 8 (CK8) is especially remarkable as it is produced early in embryogenesis, is the only type-II CK occurring in many simple epithelial cells, and can also be synthesized in certain non-epithelial cells.
Cytokeratin 8 mRNA was abundant in fetal small intestine, placenta, pancreas, lung, liver, and kidney.
Levels of cytokeratin 8 mRNA in placenta increased slightly during pregnancy.
He et al.
(2002) presented evidence that K8 is a new cytoplasmic target for JNK in Fas receptor-mediated signaling.
The functional significance of this phosphorylation could relate to regulation of JNK signaling and/or regulation of keratin dynamics.
UniProt Number:P05787
Gene ID:KRT8
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat CK8. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the N-terminal of human CK8, different from the related rat and mouse sequences by one amino acid.
