CDH5 (Cadherin 5), also known as VE-cadherin, is a type of cadherin.
It is encoded by the human gene CDH5.
Kremmidiotis et al.
(1998) mapped the human CDH5 gene to 16q22.1 using somatic cell hybrid panels.
Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions.
Therefore it was concluded that VE-cadherin serves the purpose of maintaining newly formed vessels.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 minutes is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human CDH5. Species Crossreactivity: rat. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa766-784, RFKMLAELYGSDPREELLY, at the C-terminus of human CDH5, different from the related mouse and rat sequences by three amino acids. Species Sequence Homology: mouse.
