Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase , the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation.
The latter function is carried out by a specific phosphatase, CDC25.
At least 3 human CDC25 genes code for the A, B, and C forms of CDC25.
CDC25B is mapped to 20p13.
P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation.
Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation.
In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and CDC25C at serine-216; phosphorylation of these residues is required for binding to 14-3-3 proteins .
In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation.
Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.
UniProt Number:P30305
Gene ID:CDC25B
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human and rat CDC25B. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human CDC25B, different from the related mouse sequence by three amino acids
