CCR10, C-C chemokine receptor type 10 is a protein that in humans is encoded by the CCR10 gene.
CCR10 is constitutively expressed in skin melanocytes, fibroblasts, and microvascular endothelial cells, and that it is upregulated by IL1B and TNF.
Expression was also detected in T cells, Langerhans cells, and peripheral blood mononuclear cells but not in dendritic cells.By fluorescence in situ hybridization, the GPR2 gene is mapped to 17q21.1-q21.3.
CCR10 is the receptor for CCL27.
CCR10-CCL27 interactions are involved in T cell-mediated skin inflammation.
expression of CCL27 in normal keratinocytes and its strong upregulation in skin lesions of atopic dermatitis, contact dermatitis, and psoriasis patients.
CCR10+ T lymphocytes were detected in lesional but not normal skin of these patients.
Flow cytometric analysis showed that CCL27 binds extracellular matrix components and dermal microvascular endothelial cells and fibroblasts and mediates adhesion and transendothelial migration of CCR10+ circulating leukocytes.
CCR10 is predominantly expressed on CD4+CLA+ (cutaneous lymphocyte antigen), rather than CD8+, circulating T cells.
RT-PCR, confocal microscopy, and ELISA analysis indicated that keratinocytes exposed to TNF or IL1B but not to IL4 or IFNG in vitro express increased CCL27.
UniProt Number:P46092
Gene ID:CCR10
Applications:Suitable for use in Western Blot.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat CCR10. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human CCR10, different from the related rat and mouse sequences by one amino acid.
