Caspase 14 is an enzyme that in humans is encoded by the CASP14 gene.
CASP14 belongs the evolutionarily conserved caspase family.
CASP14 was assigned to chromosome 19p13.1.
Using Northern blot and semiquantitative RT-PCR analyses, it was showed that CASP14 was transcriptionally upregulated during maintenance of confluent cultures of human epidermal keratinocytes.
The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier.
Caspase 14 seems to play a role in keratinocyte differentiation and cornification and probably regulates maturation of the epidermis by proteolytically processing filaggrin.
UniProt Number:P31944
Gene ID:CASP14
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human,mouse and rat CASP14. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human CASP14, different from the related rat and mouse sequences by four amino acids.
