Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene.
Calpain is an intracellular protease that requires calcium for its catalytic activity.
Two isozymes, calpain I (mu-calpain) and calpain II (m-calpain), with different calcium requirements, have been identified.
Both are heterodimers composed of L (large, catalytic, 80 kD) and S (small, regulatory, 30 kD) subunits.
The isozymes share an identical S subunit, with the differences arising from the L subunits, L1 (CAPN1) and L2.
By quantitative RT-PCR, Ueyama et al.
(1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy (e.g., DMD; 310200) and 2 men and 3 women with amyotrophic lateral sclerosis (ALS; 105400) compared with controls.
Using cDNA clones as probes, Ohno et al.
(1989, 1990) assign CANPL1 to chromosome 11.
UniProt Number:P07384
Gene ID:CAPN1
Applications:Suitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen), and Immunocytochemistry.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat Calpain-1. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence in the middle region of human Calpain-1 , different from the mouse sequence by two amino acids.
