BAG family molecular chaperone regulator 3(BAG3) is a member of a conserved family of cyto-protective proteins that bind to and regulate Hsp70 family molecular chaperones.
BAG3 mutations are responsible for familial dilated cardiomyopathy.
BAG3 polymorphisms are also associated with sporadic forms of the disease together with HSPB7 locus.
In muscle cells, BAG3 cooperates with the molecular chaperones Hsc70 and HspB8 to induce the degradation of mechanically damaged cytoskeleton components in lysosomes.
This process is called chaperone-assisted selective autophagy (CASA) and is essential for maintaining muscle activity in flies, mice and men.
UniProt Number:O95817
Gene ID:BAG3
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human,mouse and rat BAG3. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human BAG3, identical to the related mouse and rat sequences.
