AQP4 (Aquaporin 4), also known as MERCURIAL-INSENSITIVE WATER CHANNEL; MIWC, is protein which in humans is encoded by the AQP4 gene.
The aquaporins are a family of water-selective membrane channels found in animals, plants, and microorganisms.
AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis.
By fluorescence in situ hybridization, Lu et al.
(1996) determined that the AQP4 gene maps to 18q11.2-q12.1.
By interspecific backcross analysis, Turtzo et al.
(1997) mapped the mouse Aqp4 gene to the proximal region of chromosome 18.
Analyzing the expression of AQP4 in mammalian skeletal muscle, Frigeri et al.
(1998) found that, in immunohistochemical experiments, affinity-purified AQP4 antibodies stained selectively the sarcolemma of fast-twitch fibers.
Immunocytochemistry revealed strong AQP4 water channel expression in Muller cells in mouse retina and in fibrous astrocytes in optic nerve.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml Immunohistochemistry (Paraffin): 0.5-1.0ug/ml by heat.
Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 min is required for staining of formalin/parffin sections.
Optimal dilutions to be determined by the researcher.
Storage and Stability: Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human AQP4 under reducing conditions. Species Crossreactivity: rat. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：A synthetic peptide corresponding to aa258-274 from the C-terminal of human AQP4, different from the related rat and mouse sequences by two amino acids. UniProt: P55087. Gene ID:AQP4. Species sequence homology: mouse
