AQP3, Aquaporin 3, is the third member of the major intrinsic protein family from rat kidney.
AQP3 is localized at the basal lateral membranes of collecting duct cells in the kidney.
AQP3 appears to exist as a single copy and to comprise 6 exons distributed over 7kb.
The AQP3 gene is mapped to 9p21-p12 by fluorescence in situ hybridization.
AQP3 works as water-channel.
And it facilitates the transport of nonionic small solutes such as urea and glycerol, albeit to a smaller degree.
The results suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms.By Western blot analysis and immunofluorescence microscopy of epidermis, Sougrat et al.
(2002) showed strong expression of a ~33-kD glycosylated AQP3 protein in keratinocyte plasma membranes, 1 layer below the unstained stratum corneum (SC).
AQP3provides a short circuit for water, or water-clamp, between the base of the epidermis and the SC in order to maintain a constant water content and to prevent the formation of a continuous water gradient across the epidermis below the SC.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 minutes is required for the staining of formalin/paraffin sections
Immunocytochemistry: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human AQP3. Species Crossreactivity: mouse and rat AQP3. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human AQP3, different from the related rat and mouse sequences by one amino acid.
