Ki-67(Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene.
From study of a panel of human-rodent somatic cell hybrids, Schonk et al.
(1989) demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10.
By in situ hybridization, Fonatsch et al.
(1991) regionalized the MKI67 gene to chromosome 10q25-qter.
By FISH, Traut et al.
(1998) mapped the mouse Mki67 gene to chromosome 7F3-F5.
Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation.
Furthermore it is associated with ribosomal RNA transcription.
Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
UniProt Number:P46013
Gene ID:MKI67
Applications:Suitable for use in Western Blot.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human KI67. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human MKI67.
