Using in conjunction with T9162-72A, as a detection antibody, the detection limit of recombinant human sTNF Receptor II is ~0.2-0.4ng/well
Western Blot: 0.1-0.2ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant human sTNF-receptor II is 1.5-3ng/lane, under either reducing or non-reducing conditions
Immunohistochemistry: 3ug/ml incubated overnight at 4°C followed by a fluorescent labeled secondary antibody.
Stained PC-3 and CaCo-2 cells
Neutralization: 15-20ug/ml yields one-half maximal inhibition of the biological activity of Human sTNF Receptor-II (0.5ug/ml)
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Specificity：Recognizes human sTNF Receptor Type II.
Isotype：IgG
Calc Applications Abbrev：E IHC N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human sTNF Receptor Type II, expressed in E. coli.
