Cytokine that contributes to the inflammatory response in vivo.
Applications:Suitable for use in ELISA, Western Blot and Neutralization.
Other applications have not been tested.
Recommended Dilutions:ELISA (Sandwich): 0.5-2ug/ml using 100ul/well antibody solution) In conjunction with biotinylated anti-human IL22 (I8443-18H) as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant human IL22
Western Blot: 0.1-0.2ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-22 is 1.5-3ng/lane, under either reducing or non-reducing conditions
Neutralization: 0.4ug/ml yields one-half maximal inhibition of the biological activity of human IL-22 (3ng/ml)
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Specificity：Recognizes human IL22.
Isotype：IgG
Calc Applications Abbrev：E N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human IL22 expressed in E. coli.
