Human IGF-BP1 is a cysteine-rich secreted protein expressed in liver, decidual, and kidneys and is the most abundant IGF-BP in amniotic fluid.
Levels of IGF-BP1 in serum are lowest after food.
IGF-BP1 binds both IGF-I and IGF-II with equal affinity.
Phosphorylated IGF-BP1 hinders IGF actions, whereas nonphosphorylated IGF-BP1 is stimulatory.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Sandwich ELISA: 0.5-2ug/ml using 100ul/well
Western Blot: 0.1-0.2ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP1 is 1.5-3ng/lane, under either reducing or non-reducing conditions
Immunohistochemistry (FFPE): 0.125-0.2ug/ml using human normal placenta incubated overnight at 4˚C.
An HRP-labeled polymer detection system was used with a DAB chromogen.
It is recommended that heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is used
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography. Endotoxin: <0.1ng/ug protein (<1EU/ug)
Form：Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Specificity：Recognizes human IGFBP1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human IGFBP1 expressed in E. coli.
