84-2092-28　Heregulin beta-1 (Neuregulin-1, Acetylcholine Receptor-inducing Activity, Breast Cancer Cell Differentiation Factor P45, Glial Growth Factor, Heregulin, Neu Differentiation Factor, Sensory and Motor Neuron-derived Factor) 100ug　143581

特徴

• Neuregulin 1 (NRG1) was originally identified as a 44kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues.
• It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing.
• These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF).
• They are tissue-specifically expressed and differ significantly in their structure.
• The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains.
• GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms.
• The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors.
• Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Neutralization.
• Other applications not tested.
• Recommended Dilutions:ELISA (Sandwich): 0.5-2ug/ml detects human Heregulin beta using 100ul/well antibody solution.
• The detection limit is at least 0.2-0.4ng/well for recombinant human Heregulin beta-1Western Blot: 0.1-0.2ug/ml.
• The detection limit for recombinant human Heregulin beta1 is 1.5-3ng/lane, under either reducing or non-reducing conditions
• Immunohistochemistry (FFPE): 0.0625-0.125mg/ml with an overnight incubation at 4°C stains formalin-fixed, paraffin-embedded sections of human normal breast.
• Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.
• Neutralization: 0.7-1.1ug/ml yields one-half maximal inhibition of the biological activity of human Heregulin beta1 (1ng/ml)
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.