VIME is a member of the intermediate filament family.
Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton.
This protein is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions.
It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification.
It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling.
Applications:Suitable for use in Flow Cytometry (Not Tested), Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:Flow Cytometry (Not Tested): 1:10-1:50Immunohistochemistry: 1:10-1:50Western Blot: 1:1,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography, followed by peptide affintiy purifcation.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human Vimentin.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Vimentin
