Covalent modification of target lysines by SUMO (small ubiquitin-like modifier) modulates processes such as protein localization, transcription, nuclear transport, mitosis, DNA replication and repair, signal transduction, and viral reproduction.
SUMO does not seem to be involved in protein degradation and may in fact function as an antagonist of ubiquitin in the degradation process.
In the development of Drosophila, SUMO plays a maternal role in anterior-posterior (A/P) polarity and patterning.
Applications:Suitable for use in FLISA and Western Blot.
Other applications not tested.
Recommended Dilutions:FLISA: 1:1000 Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:200ul
Host:rabbit
Source Antibody:drosophila
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity:Recognizes Drosophila SUMO. Species Crossreactivity: human
Isotype:IgG
Calc Applications Abbrev:FLISA WB
Calc Crossreactivity:Dr Hu
Immunogen:Synthetic peptide selected from the C-terminal region of Drosophila SUMO (KLH).