Stk38,also known as NUCLEAR DBF2-RELATED PROTEIN, contains 1 protein kinase domain that interacts with mob1 and mob2.The homodimeric s100b binds to two molecules of stk38.
It is ubiquitously expressed with highest levels observed in peripheral blood leukocytes.
Stk38 activated by binding of s100b which releases autoinhibitory n-lobe interactions, enabling atp to bind and the autophosphorylation of ser-281.
thr-444 then undergoes calcium- dependent phosphorylation by an upstream kinase.
Interactions between phosphorylated thr-444 and the n-lobe promote additional structural changes that complete the activation of the kinase.
Autoinhibition is also released by the binding of mob1/mobkl1a and mob2/hcca2 to the n-terminal of stk38.
Applications:Suitable for use in FLISA and Western Blot.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000 Western Blot: 1:250-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human STK38.
Isotype：IgG
Calc Applications Abbrev：FLISA WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide selected from the C-terminal region of human STK38 (KLH).
